LOCUS       pUC19-promKanprom        4334 bp ds-DNA     circular     30-AUG-2016
DEFINITION  Cloning vector pUC19-promKanprom
ACCESSION   xxxxxx
SOURCE      pUC19-promKanprom
  ORGANISM  Cloning vector pUC19-promKanprom other sequences; artificial sequences;
            vectors.
REFERENCE   1
  AUTHORS   McLean,T.C., Hoskisson,P.A. and Seipke,R.F.
  TITLE     Coordinate regulation of antimycin and candicidin biosynthesis
  JOURNAL   mSphere 1:e00305-16 (2016)
FEATURES             Location/Qualifiers
     CDS             201..1061
                     /label="bla"
     misc_feature    complement(2292..2584)
                     /label="ermE*p"
     misc_feature    complement(2295..2294)
                     /label="ATG start codon"
     primer_bind     2585..2604
                     /Sequence="ATTCCGGGGATCCGTCGACC"
                     /label="Redirect P1"
     misc_signal     complement(2613..2646)
                     /label="FRT"
     CDS             complement(2647..3441)
                     /label="neo/kan"
     misc_signal     complement(3582..3615)
                     /label="FRT"
     primer_bind     complement(3616..3634)
                     /Sequence="TGTAGGCTGGAGCTGCTTC"
                     /label="Redirect P2"
     misc_feature    3635..3936
                     /modified_by="User"
                     /label="rpsL(XC)p"
     misc_feature    3937..3939
                     /label="ATG start codon"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg
     2281 agctcgaatt ccatatgggg cctcctgttc tagacgatcc taccaaccgg cacgattgtg
     2341 cccacaacag catcgcggtg ccacgtgtgg accgcgtcgg tcagatcctc cccgcacctc
     2401 tcgccagccg tcaagatcga ccgcgtgcac ctgcgatcgc cgatcaaccg cgactagcat
     2461 cgggcgcaag ccgccactcg aacggacact cgcatggacg tccccttcct ggacctgcaa
     2521 gccgcgtacc tcgaactccg gtccgacatc gaccaggcgt gccggcgcgt gctcgggtcg
     2581 ggctattccg gggatccgtc gacctgcagt tcgaagttcc tattctctag aaagtatagg
     2641 aacttctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc
     2701 ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat
     2761 atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc
     2821 gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg
     2881 ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc
     2941 tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat
     3001 ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg
     3061 atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc
     3121 aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc
     3181 cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga
     3241 tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa
     3301 aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt
     3361 ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg
     3421 caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga
     3481 tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt
     3541 cgctcgccag tcgattggct gagctcataa gttcctattc cgaagttcct attctctaga
     3601 aagtatagga acttcgaagc agctccagcc tacagccctg caggcggaag tcaggtagac
     3661 acgacttccg ctagtccttg caaggtctgc tgacgtgagg cggggcggtc gtttttgacc
     3721 gccctgcctt cgtcatgtag gctcgctcgc tgtgcctggc gtgtcatcag acgcccaggt
     3781 cccggtgccg tgaggcccgg gccatcgagc cggtggtacg tggctgcggt ccccttgtga
     3841 gggctgcgcg ccgtgtgctg tccggcgcgc acagccttga atccacccgc gggggccggc
     3901 cggtctccgt gagctcgagt agacgacgga gacgtaatga ctggccgtcg ttttacaacg
     3961 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4021 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4081 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4141 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4201 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4261 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4321 accgaaacgc gcga
//